Crop Resilience: Genetic Engineering and CRISPR in Agriculture

Authors

DOI:

https://doi.org/10.55938/wlp.v1i2.115

Keywords:

CRISPR-Cas9, Precision Breeding, DNA Sequencing, Plant Microbiomes, Bioinformatics

Abstract

The study of plant-microbe interactions, disease resistance, and activities that promote plant development is increasing attention in the application of CRISPR/Cas genome editing techniques. Their potential utilization in agriculture and their possible effects on plant health are examined in this review. Food security and agricultural improvement are vastly enhanced by genome editing; yet, in order to fully realize its potential, certain challenges and constraints must be overcome. Utilizing less pesticides and increasing crop yield, gene-editing technologies, especially CRISPR-Cas9 systems, have produced crops that are resistant to disease. This analysis emphasizes the function of genome editing in sustainable agriculture through CRISPR-Cas9. By reproducing plants with desired features, CRISPR/Cas9 technology transforms genome engineering. It is utilized by the agricultural sectors to strengthen quality, productivity, resistance to disease, and stress. CRISPR/Cas-based gene knockdown facilitates crop domestication and hybrid breeding while improving yield, quality, and resistance. The speed, accuracy, and cost of CRISPR technology make it a promising tool for revolutionizing agricultural biotechnology. Its creations, including as disease-resistant wheat, drought-tolerant cereals, and nutrient-efficient maize, have been leveraged to improve crop performance and address global food security. However, barriers including societal perception, technological constraints, legal limitations, and ethical dilemmas prevent its widespread adoption. Innovations in technology make it possible to domesticate wild plants from scratch, which supports sustainable agriculture and food security. Future agricultural growth depends on our ability to comprehend essential domestication genes and manipulate target sequences precisely.

References

1. Munawar, S., ul Qamar, M. T., Mustafa, G., Khan, M. S., & Joyia, F. A. (2020). Role of biotechnology in climate resilient agriculture. Environment, climate, plant and vegetation growth, 339-365.

2. Rogo, U., Simoni, S., Fambrini, M., Giordani, T., Pugliesi, C., & Mascagni, F. (2024). Future-Proofing Agriculture: De Novo Domestication for Sustainable and Resilient Crops. International Journal of Molecular Sciences, 25(4), 2374.

3. Yadav, R. K., Tripathi, M. K., Tiwari, S., Tripathi, N., Asati, R., Chauhan, S., ... &Payasi, D. K. (2023). Genome editing and improvement of abiotic stress tolerance in crop plants. Life, 13(7), 1456.

4. Munaweera, T. I. K., Jayawardana, N. U., Rajaratnam, R., & Dissanayake, N. (2022). Modern plant biotechnology as a strategy in addressing climate change and attaining food security. Agriculture & Food Security, 11(1), 1-28.

5. Wray-Cahen, D., Bodnar, A., Rexroad, C., Siewerdt, F., & Kovich, D. (2022). Advancing genome editing to improve the sustainability and resiliency of animal agriculture. CABI Agriculture and Bioscience, 3(1), 1-17.

6. Roy, A., Purkaystha, S., & Bhattacharyya, S. (2021). Advancement in Molecular and Fast Breeding Programs for Climate-Resilient Agriculture Practices. Harsh Environment and Plant Resilience: Molecular and Functional Aspects, 73-98.

7. Shelake, R. M., Pramanik, D., & Kim, J. Y. (2019). Exploration of plant-microbe interactions for sustainable agriculture in CRISPR era. Microorganisms, 7(8), 269.

8. Ijaz, M., Khan, F., Ahmed, T., Noman, M., Zulfiqar, F., Rizwan, M., ... & Li, B. (2023). Nanobiotechnology to advance stress resilience in plants: Current opportunities and challenges. Materials Today Bio, 100759.

9. Shendekar, S., Mangla, S., Gore, V., Meshram, M., & Raut, D. (2024). Chapter-4 The CRISPR Revolution: Editing Plant Genomes for Abiotic Stress Resilience and a Better Tomorrow. Biotechnology and Biological Sciences, 91.

10. Ricroch, A., Clairand, P., & Harwood, W. (2017). Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture. Emerging Topics in Life Sciences, 1(2), 169-182.

11. Chen, K., Wang, Y., Zhang, R., Zhang, H., & Gao, C. (2019). CRISPR/Cas genome editing and precision plant breeding in agriculture. Annual review of plant biology, 70, 667-697.

12. Baltes, N. J., Gil-Humanes, J., &Voytas, D. F. (2017). Genome engineering and agriculture: opportunities and challenges. Progress in molecular biology and translational science, 149, 1-26.

13. Saad, N. S. M., Neik, T. X., Thomas, W. J., Amas, J. C., Cantila, A. Y., Craig, R. J., ... & Batley, J. (2022). Advancing designer crops for climate resilience through an integrated genomics approach. Current Opinion in Plant Biology, 67, 102220.

14. Ali, Q., Yu, C., Hussain, A., Ali, M., Ahmar, S., Sohail, M. A., ... & Zhou, L. (2022). Genome engineering technology for durable disease resistance: Recent progress and future outlooks for sustainable agriculture. Frontiers in Plant Science, 13, 860281.

15. Rao, M. J., & Wang, L. (2021). CRISPR/Cas9 technology for improving agronomic traits and future prospective in agriculture. Planta, 254, 1-16.

16. Sampath, V., Rangarajan, N., CH, S., Deori, M., Veeraragavan, M., Ghodake, B. D., & Kaushal, K. (2023). Advancing Crop Improvement Through CRISPR Technology in Precision Agriculture Trends-A Review. International Journal of Environment and Climate Change, 13(11), 4683-4694.

17. Shendekar, S., Mangla, S., Gore, V., Meshram, M., & Raut, D. (2024). The CRISPR Revolution: Editing Plant Genomes for Abiotic Stress Resilience and a Better Tomorrow. Biotechnology and Biological Sciences, 93.

18. Sarfraz, S., Ali, F., Hameed, A., Ahmad, Z., & Riaz, K. (2023). Sustainable Agriculture through Technological Innovations. In Sustainable Agriculture in the Era of the OMICs Revolution (pp. 223-239). Cham: Springer International Publishing.

19. Kaul, T., Sony, S. K., Bharti, J., Motelb, K. F. A., Verma, R., Thangaraj, A., ... & Eswaran, M. (2022). CRISPR genome editing brings global food security into the first Lane: Enhancing nutrition and stress resilience in crops. In Next-generation plant breeding approaches for stress resilience in cereal crops (pp. 285-344). Singapore: Springer Nature Singapore.

20. Zhu, H., Li, C., & Gao, C. (2020). Applications of CRISPR–Cas in agriculture and plant biotechnology. Nature Reviews Molecular Cell Biology, 21(11), 661-677.

21. Mehta, S., Lal, S. K., Sahu, K. P., Venkatapuram, A. K., Kumar, M., Sheri, V., ... & Reddy, M. K. (2020). CRISPR/Cas9-edited rice: a new frontier for sustainable agriculture. New frontiers in stress management for durable agriculture, 427-458.

22. Demirer, G. S., Silva, T. N., Jackson, C. T., Thomas, J. B., W. Ehrhardt, D., Rhee, S. Y., ... & Landry, M. P. (2021). Nanotechnology to advance CRISPR–Cas genetic engineering of plants. Nature Nanotechnology, 16(3), 243-250.

23. Ahmad, M. (2023). Plant breeding advancements with “CRISPR-Cas” genome editing technologies will assist future food security. Frontiers in Plant Science, 14, 1133036.

24. Camerlengo, F., Frittelli, A., &Pagliarello, R. (2022). CRISPR towards a Sustainable Agriculture. Encyclopedia, 2(1), 538-558.

25. Somashekar, H. (2024). Harnessing Genome Editing Approaches in Crop Improvement to Reshape Modern Agriculture. In Smart Breeding (pp. 105-122). Apple Academic Press.

26. Albahri, G., Alyamani, A. A., Badran, A., Hijazi, A., Nasser, M., Maresca, M., & Baydoun, E. (2023). Enhancing essential grains yield for sustainable food security and bio-safe agriculture through latest innovative approaches. Agronomy, 13(7), 1709.

27. Tan, C., Kalhoro, M. T., Faqir, Y., Ma, J., Osei, M. D., & Khaliq, G. (2022). Climate-Resilient Microbial Biotechnology: A Perspective on Sustainable Agriculture. Sustainability, 14(9), 5574.

Published

2024-11-21

How to Cite

Thapliyal, S., Bisht, K., & Kaur, J. (2024). Crop Resilience: Genetic Engineering and CRISPR in Agriculture. Wisdom Leaf Press, 1(2), 71–77. https://doi.org/10.55938/wlp.v1i2.115

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